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The effect of increase in naci concentration on growth and proline content of purple yam (dioscorea alata l.) Grown in vitro

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Tuber of purple yarn (Dioscorea alata L.) has been used as an alternative food in some areas in Indonesia. The tuber contains high carbohydrate, low glycemic index and gluten free, therefore, study on genetic improvement of this species is needed to increase the productivity and to find out new cultivars which can be cultivated in marginal lands. This research was aimed to investigate the effect of NaC1 concentration on growth and proline content of purple yam grown in vitro. Shoot tips were cultured on MS (Murashige and Skoog) medium supplemented with NaCl at concentrations of 25, 50, 100, 200 and 250 mM. After six weeks in culture, height of shoots, number of nodes, number of leaves, as well as proline content were recorded. The results showed that shoots grown on MS medium supplemented with NaCI at 25 and 50 mM had better growth compared to control. The best medium for its growth was MS containing 50 mM of NaCI. Increase in NaCI level’s resulted in decrease of growth. The LD50 value was obtained at 1 83 mM of NaCI. The highest proline concentration was achieved by shoots grown on the medium supplemented with 100 mM of NaCI. This result indicated that purple yam was tolerant to the increase of NaCI concentration up to 100 mM, on MS medium without addition of plant growth regulators.

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Ketersediaan

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Informasi Detail

Judul Seri

Annales bogorienses

No. Panggil

-

Penerbit

: Prentice Limited., 2012

Deskripsi Fisik

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Bahasa

English

ISBN/ISSN

0517-8452

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

Vol. 16, No. 2, Tahun

Subjek

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Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

Lampiran Berkas

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