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Improved regeneration, acclimatization and shoot cutting production of “Gebang” cassava derived from irradiated in vitro shoots

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Gebang is an Indonesian local genotype which has been selected as superior genotype for low amylose cassava. Prior to induction of new mutants of this genotype, series of research have been conducted to improve regeneration and acclimatization as well as shoot cuttings production of irradiated in vitro shoots. Four dosage treatments of gamma ray irradiation i.e. 0, 0.2, 1 .0, 1 .5, 2.0 krad were applied to 32 in vitro shoots multiplied from apical shoots of plants in the field. The highest multiplication rate and acclimatized shoots were obtained from shoots irradiated with 0.2 krad multiplied on various level of BAP added on MS medium and resulted that MS supplemented with I 1 mg/L BAP was the best medium. The phenotypic variation was observed in shoots irradiated with 1 .0, 1 .5 and 2.0 krad. while that irradiated with 0.2 krad performed normal appearances. Subsequent production of propagated young stem cuttings so called “ratooning system” decreased after the second cycle of propagation especially in the survival rate of that irradiated with 0.2 krad. The findings lead to the opportunity to produce cassava propagules derived from irradiated in vitro culture at a higher amount using Gebang genotype as a model

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Ketersediaan

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Informasi Detail

Judul Seri

Annales bogorienses

No. Panggil

-

Penerbit

: Prentice Limited., 2012

Deskripsi Fisik

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Bahasa

English

ISBN/ISSN

0517-8452

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

Vol. 16, No. 2, Tahun

Subjek

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Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

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