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Polyaromatic Hydrocarbon Degradation and Dioxygenase Gene Detection from Alteromonas alvinellae BtO5

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Bt05 is marine bacterium which was isolated From the Jakarta flay, Indonesia. The aim of this study was to characterize PAHs-degrading property and molecular identification by partial analysis of 16S rRNA gene, and to partially analyze dioxygenase gene of 13105 isolate. Our further study on this isolate revealed that it could degrade three PAHs (phenanthrene, dibenzothiophene, fluorene) between 60%-90% within 11 days at 100 ppm level. This finding indicated the potential of the isolate for bioremediation of PAHs. The isolate was identified as Alteromonas alvinellae by phylogenetic analysis of I 6S rRNA gene sequence. Sequence analysis of the PCR product of PA I-I dioxygenase genes amplified using two primer set (iiDA and ppAH) of the isolate were identified 97% as naphthalene dioxygenase gene (phaAc) and 58% as I ,2-dioxygenase.

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Ketersediaan

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Informasi Detail

Judul Seri

Annales bogorienses

No. Panggil

660.6 Ann

Penerbit

: Prentice Limited., 2013

Deskripsi Fisik

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Bahasa

English

ISBN/ISSN

0517-8452

Klasifikasi

660.6

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

Vol. 17, No. 1, Tahun

Subjek

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Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

Lampiran Berkas

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