Identification of differentially expressed cDNA in cassava under drought stress using cDNA-RAPD approach

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Identification of differentially expressed cDNA in cassava under drought stress using cDNA-RAPD approach

Salam, Haipan - Konferensi; Herlinawati - Konferensi; Afidah - Konferensi; Sunani, Nuning Hidayah - Konferensi;

Cassava is an important carbohydrate source that provides food security and alternative renewable energy development. This plant is naturally drought tolerant, but there is a wide variation within cassava genotypes in their ability to maintain high yield and starch production under drought stress. It has been reported that cassava clones with leaf retention or stay- green trait can produce more total fresh biomass and high root dry matter compared to drought susceptible cultivars. The genetic. biochemical and molecular bases of stay-green trait, needs to be understood in order to develop drought resistant cassava cultivars since water stress limits yield and starch production. Differential Display (DD) RT-PCR is a powerful technique for analyzing differences in gene expression. The method is based on the detection of the differentially expressed cDNAs out of two or more samples. The main objective of this study was to identify differentially expressed cDNA in cassava under drought stress by employing a cDNA-RAPD approach. In this study, we used cassava genotype Ubi Kuning which was considered the most responsive to water insufficiency (45 days without watering). Leaf samples were collected from water-stressed and well-watered plants at day 45. Among ii random primers, OPBO3 and OPH17 have identified differentially expressed cDNA in Ubi Kuning. Further characterization of these PCR products of expressed cDNA under drought stress may open possibility of the development of cassava with improved drought resistance through genetic engineering and/or marker assisted selection (MAS).

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Judul Seri: Annales bogorienses
No. Panggil: 660.6 Ann
Penerbit: : Prentice Limited., 2013
Deskripsi Fisik: -
Bahasa: English
ISBN/ISSN: 0517-8452
Klasifikasi: 660.6
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: Vol. 17, No. 1, Tahun
Subjek: -
Info Detail Spesifik: -
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